Mouse Blood Collection

Blood Collection Biomethodology For Laboratory Mice Sampling will occur every 2 weeks, up to 10% of the total blood volume may be drawn or 8 µl g (4). for a 25 g mouse, this is equivalent to 200 µl every 2 weeks. for repeated blood collection, fluid replacement does not substitute for a larger blood volume or more frequent blood collection. example for a 25 g mouse* single sample . without fluid. Allow drop to take affect and then wipe away any excess fluid. place a hematocrit tube at the medial canthus of the eye (as shown in bottom illustration). with a rotating motion, insert tube through the conjunctiva membrane. continue rotating the tube until blood flows. collect blood into an appropriate container.

Blood Collection Biomethodology For Laboratory Mice The approximate circulating blood volume (cbv or total blood volume tbv) of adult rodents varies with species and body weight [mouse 63 to 80 ml kg (mean 72 ml kg) and rat 58 70 ml kg (mean 64 ml kg)].3 of the circulating blood volume, approximately 10% of the total volume can be safely removed every 2 to 4 weeks, 7.5% every 7 days, and 1%. Restrain a conscious mouse by the scruff and use a lancet of appropriate size to puncture the vein, slightly behind the mandible and in front of the ear canal. after the vein is punctured, collect blood in an appropriate tube. then, if necessary, apply pressure with a gauze sponge until the bleeding stops. Tail vein up to 0.2 ml following collection site cleaning with 70% alcohol, the mouse is restrained (state method of restraint) and blood is collected using a 25 27 gauge needle from the lateral tail vein. blood flow is stopped by applying pressure with sterile gauze to achieve hemostasis. • one or two blood samples can be taken per session. The animal and the red blood cell (rbc) turnover rate.ǂ the approximate circulating blood volume of adult rodents varies with species and body weight (mouse 63 to 80 ml kg (mean 72 ml kg) and rat 58 70 ml kg (mean 64 ml kg)).4 of the circulating blood volume, approximately 10% of the total volume can be safely.

Submandibular Blood Collection In Mice Animals In Science Tail vein up to 0.2 ml following collection site cleaning with 70% alcohol, the mouse is restrained (state method of restraint) and blood is collected using a 25 27 gauge needle from the lateral tail vein. blood flow is stopped by applying pressure with sterile gauze to achieve hemostasis. • one or two blood samples can be taken per session. The animal and the red blood cell (rbc) turnover rate.ǂ the approximate circulating blood volume of adult rodents varies with species and body weight (mouse 63 to 80 ml kg (mean 72 ml kg) and rat 58 70 ml kg (mean 64 ml kg)).4 of the circulating blood volume, approximately 10% of the total volume can be safely. The tail vein can be used for repeated or serial blood collection. anesthesia. this method can be performed without anesthesia when using a restraint device. procedure. restrain the animal using the mechanical restraint device of your choice with the tail protruding. visualize anatomic landmarks (figures 1 and 2). Here we describe recommended techniques for blood collection from mice and the considerations that have to be taken into account to get adequate samples for hematological investigations. furthermore, we describe established methods used in the german mouse clinic (gmc) to determine hematological parameters in the mouse. curr.

Blood Collection Biomethodology For Laboratory Mice The tail vein can be used for repeated or serial blood collection. anesthesia. this method can be performed without anesthesia when using a restraint device. procedure. restrain the animal using the mechanical restraint device of your choice with the tail protruding. visualize anatomic landmarks (figures 1 and 2). Here we describe recommended techniques for blood collection from mice and the considerations that have to be taken into account to get adequate samples for hematological investigations. furthermore, we describe established methods used in the german mouse clinic (gmc) to determine hematological parameters in the mouse. curr.

Mouse Blood Collection